| 质粒名称: | pLVX-Tight-Puro FLAG-SMAD4 TetON |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107129 |
| 存储实验室: | Joan Massague |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX tight puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SMAD4 |
| 物种来源: | -- |
| 插入大小: | 1700 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | FLAG |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI（销毁后未知） |
| 3' 克隆位点: | EcoRI（销毁后未知） |
| 5' 测序引物 | BamHI |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-Tight-Puro FLAG-SMAD4 TetON was a gift from Joan Massague (Addgene plasmid # 107129 ; http://n2t.net/addgene:107129 ; RRID:Addgene_107129) |
| 参考文献部分: | TGF-beta Tumor Suppression through a Lethal EMT. David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, Iacobuzio-Donahue CA, Massague J. Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009. Epub 2016 Feb 18. 10.1016/j.cell.2016.01.009 PubMed 26898331 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-Tight-Puro FLAG-SMAD4 TetON 9403 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Inducible expression of Flag-tagged SMAD4 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pLVX-Tight-Puro FLAG-SMAD4 TetON
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9403)
AUTHORS David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N,
Iacobuzio-Donahue CA, Massague J
TITLE TGF-beta Tumor Suppression through a Lethal EMT.
JOURNAL Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009.
Epub 2016 Feb 18.
PUBMED 26898331
REFERENCE 2 (bases 1 to 9403)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9403
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 680..805
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1302..1535
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2027..2142
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2205..2519
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
protein_bind 2213..2231
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2249..2267
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2284..2302
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2320..2338
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2356..2374
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2391..2409
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2427..2445
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 2492..2516
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2533..2542
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2542..2565
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
promoter 4235..4734
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4270..4293)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4667..4686
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 4755..5354
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4755..4774)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5251..5271
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5371..5959
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5424..5444)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5842..5853)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6166..6799
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6927..6943)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6927..6943)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6940..6962)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6951..6967
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6975..7005)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7020..7041
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7158..7175)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7329..7917)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7409..7428)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8088..8948)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8711..8730
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8949..9053)
/gene="bla"
/label=AmpR promoter
polyA_signal 9101..9235
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9138..9157)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9192..9211
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 9403..633
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagttt actccctatc
2221 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
2281 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
2341 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
2401 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
2461 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
2521 ggagaaggat ccgccaccat ggattacaag gacgacgatg acaagatgga caatatgtct
2581 attacgaata caccaacaag taatgatgcc tgtctgagca ttgtgcatag tttgatgtgc
2641 catagacaag gtggagagag tgaaacattt gcaaaaagag caattgaaag tttggtaaag
2701 aagctgaagg agaaaaaaga tgaattggat tctttaataa cagctataac tacaaatgga
2761 gctcatccta gtaaatgtgt taccatacag agaacattgg atgggaggct tcaggtggct
2821 ggtcggaaag gatttcctca tgtgatctat gcccgtctct ggaggtggcc tgatcttcac
2881 aaaaatgaac taaaacatgt taaatattgt cagtatgcgt ttgacttaaa atgtgatagt
2941 gtctgtgtga atccatatca ctacgaacga gttgtatcac ctggaattga tctctcagga
3001 ttaacactgc agagtaatgc tccatcaagt atgatggtga aggatgaata tgtgcatgac
3061 tttgagggac agccatcgtt gtccactgaa ggacattcaa ttcaaaccat ccagcatcca
3121 ccaagtaatc gtgcatcgac agagacatac agcaccccag ctctgttagc cccatctgag
3181 tctaatgcta ccagcactgc caactttccc aacattcctg tggcttccac aagtcagcct
3241 gccagtatac tggggggcag ccatagtgaa ggactgttgc agatagcatc agggcctcag
3301 ccaggacagc agcagaatgg atttactggt cagccagcta cttaccatca taacagcact
3361 accacctgga ctggaagtag gactgcacca tacacaccta atttgcctca ccaccaaaac
3421 ggccatcttc agcaccaccc gcctatgccg ccccatcccg gacattactg gcctgttcac
3481 aatgagcttg cattccagcc tcccatttcc aatcatcctg ctcctgagta ttggtgttcc
3541 attgcttact ttgaaatgga tgttcaggta ggagagacat ttaaggttcc ttcaagctgc
3601 cctattgtta ctgttgatgg atacgtggac ccttctggag gagatcgctt ttgtttgggt
3661 caactctcca atgtccacag gacagaagcc attgagagag caaggttgca cataggcaaa
3721 ggtgtgcagt tggaatgtaa aggtgaaggt gatgtttggg tcaggtgcct tagtgaccac
3781 gcggtctttg tacagagtta ctacttagac agagaagctg ggcgtgcacc tggagatgct
3841 gttcataaga tctacccaag tgcatatata aaggtctttg atttgcgtca gtgtcatcga
3901 cagatgcagc agcaggcggc tactgcacaa gctgcagcag ctgcccaggc agcagccgtg
3961 gcaggaaaca tccctggccc aggatcagta ggtggaatag ctccagctat cagtctgtca
4021 gctgctgctg gaattggtgt tgatgacctt cgtcgcttat gcatactcag gatgagtttt
4081 gtgaaaggct ggggaccgga ttacccaaga cagagcatca aagaaacacc ttgctggatt
4141 gaaattcact tacaccgggc cctccagctc ctagacgaag tacttcatac catgccgatt
4201 gcagacccac aacctttaga ctgagaattc taccgggtag gggaggcgct tttcccaagg
4261 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
4321 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
4381 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
4441 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
4501 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
4561 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
4621 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
4681 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
4741 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
4801 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
4861 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
4921 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
4981 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
5041 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
5101 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
5161 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
5221 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
5281 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
5341 agcccggtgc ctgacgggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
5401 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
5461 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
5521 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
5581 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
5641 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
5701 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
5761 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
5821 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
5881 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
5941 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
6001 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
6061 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
6121 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
6181 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
6241 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
6301 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
6361 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
6421 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
6481 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
6541 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
6601 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
6661 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
6721 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
6781 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
6841 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc
6901 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
6961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
7021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
7081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
7141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
7201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
7261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
7321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
7381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
7441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
7501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
7561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
7621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
7681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
7741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
7801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
7861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
7921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
7981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
8041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
8101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
8161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
8221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
8281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
8341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
8401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
8461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
8521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
8581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
8641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
8701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
8761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
8821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
8881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
8941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
9001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
9061 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
9121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
9181 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
9241 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
9301 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
9361 aagaaattgt atttgttaaa tatgtactac aaacttagta gtt
//
